sindbis virus sinv  (ATCC)

Journal: Virus Research

Article Title: Mosquito-borne alphaviruses in Zambia: Isolation and characterization of Eilat and Sindbis viruses

doi: 10.1016/j.virusres.2025.199604

Figure Lengend Snippet: Growth kinetics of Zambian SINV isolate M115 Vero (A) and BHK-21 (B) cells were infected with SINV isolate M115 and AR339 at an MOI of 0.0001. C6/36 cells (C) were infected with SINV at an MOI of 0.01. Titers at the indicated each time point were determined by plaque assay. The values shown are mean ± standard deviation (SD) of triplicate. * p < 0.05, *** p < 0.001 by two-way ANOVA test.

Article Snippet: The SINV AR339 strain (VR-1248) was obtained from the ATCC.

Techniques: Infection, Plaque Assay, Standard Deviation

Journal: Nature Communications

Article Title: Massive multiplexing of spatially resolved single neuron projections with axonal BARseq

doi: 10.1038/s41467-024-52756-x

Figure Lengend Snippet: A Workflow. The brain is injected with barcoded viral library. After 24–48 h of expression, during which RNA barcodes are transported to axon terminals, where they are amplified into rolonies and sequenced. B , C Single rolonies in axons have significantly weaker signals compared to somatic rolonies. B Representative image of somatic and axonal rolonies; dotted circle: somatic rolonies; arrow: axonal rolonies, with zoom-in views shown on the right; rolony intensity is color coded. Scale bar: 100 µm. C Quantification of intensity between axonal and somatic rolonies. Due to the large intensity difference between somatic and axonal rolonies, proper exposure for axonal rolonies often results in saturation of somatic rolonies. Paired t -test, two-tailed, p -value < 0.0001. D Representative images of axonal and somatic rolonies in AudC/I and ipsilateral thalamus. Images are from the first cycle of in situ sequencing. Similar results are observed across sections of the same brain regions. Dotted line, anatomical boundaries. Scale bar: top, 100 µm; bottom, 25 µm. E Registered barcode signals in CCFv3. Top, data in 3D model. Gray, brain outline. Bottom, coronal view of 25 µm of the sample. Gray, DAPI. F Representative images of in situ sequencing soma and a single axonal rolony with the same barcode. Soma ROI, 30.25 µm × 30.25 µm from injection site; axonal rolony ROI, 14.85 µm × 14.85 µm from ipsilateral thalamus. In total, 17 sequencing cycles are shown. G An example of tracing tracks for a single barcoded neuron reconstructed by connecting rolonies. Rolony location is indicated in white; soma location is indicated as a large green dot in ipsilateral cortex. AudC/I, contra/ipsilateral auditory cortex; Thal, thalamus; BC, barcode; D, dorsal; V, ventral; C, caudal; R, rostral; Seq, sequencing cycle.

Article Snippet: The sindbis virus (SINV) barcode library used in this study was generated by the MAPseq core facility at Cold Spring Harbor Laboratory.

Techniques: Injection, Expressing, Amplification, Two Tailed Test, In Situ, Sequencing