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sindbis virus sinv  (ATCC)


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    ATCC sindbis virus sinv
    Sindbis Virus Sinv, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sindbis+virus+sinv/Sindbis+virus/pm41319272-42-35-50
    Average 94 stars, based on 102 article reviews
    sindbis virus sinv - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Sulforhodamine B Assay:

    Article Title: Fluorescence-based biosensors for West Nile virus detection.
    Article Snippet: .. WNV lineage 1(L1) (NY99-flamingo382-99), lineage 2 (L2) (SRB/Novi Sad/12), DENV serotype 2 (DENV-2/ 16681/PDK53), ZIKV strains from the American (PA259459), Asian (FSS13025), and African (MR766) lineages, USUV isolates (SAAR-1776 and Vienna 2001), Coxsackie B5 (CVB5), Sindbis virus (SINV) and Semliki Forest virus (SFV) were propagated and titrated on Vero-81 cells (ATCC CCL-81, Manassas, VA, USA) [15] and used under proper BSL-2 or BSL-3 containment facilities following current national legislation. .. The NS2B/NS3 protease target sites of WNV L2 (SRB/ Novi Sad/12) were fused to a fluorescence quenching peptide (dark peptide) [16] and to the fluorescent protein GFP or Neptune [17], and cloned into the eukaryotic expression vector pcDNA3.1+ (Invitrogen) using third-party commercial services (GeneScript).

    Article Title: Fluorescence-based biosensors for West Nile virus detection
    Article Snippet: .. WNV lineage 1(L1) (NY99-flamingo382-99), lineage 2 (L2) (SRB/Novi Sad/12), DENV serotype 2 (DENV-2/16681/PDK53), ZIKV strains from the American ( PA259459 ), Asian (FSS13025), and African (MR766) lineages, USUV isolates (SAAR-1776 and Vienna 2001), Coxsackie B5 (CVB5), Sindbis virus (SINV) and Semliki Forest virus (SFV) were propagated and titrated on Vero-81 cells (ATCC CCL-81, Manassas, VA, USA) [ ] and used under proper BSL-2 or BSL-3 containment facilities following current national legislation. .. The NS2B/NS3 protease target sites of WNV L2 (SRB/Novi Sad/12) were fused to a fluorescence quenching peptide (dark peptide) [ ] and to the fluorescent protein GFP or Neptune [ ], and cloned into the eukaryotic expression vector pcDNA3.1+ (Invitrogen) using third-party commercial services (GeneScript).

    Virus:

    Article Title: Fluorescence-based biosensors for West Nile virus detection.
    Article Snippet: .. WNV lineage 1(L1) (NY99-flamingo382-99), lineage 2 (L2) (SRB/Novi Sad/12), DENV serotype 2 (DENV-2/ 16681/PDK53), ZIKV strains from the American (PA259459), Asian (FSS13025), and African (MR766) lineages, USUV isolates (SAAR-1776 and Vienna 2001), Coxsackie B5 (CVB5), Sindbis virus (SINV) and Semliki Forest virus (SFV) were propagated and titrated on Vero-81 cells (ATCC CCL-81, Manassas, VA, USA) [15] and used under proper BSL-2 or BSL-3 containment facilities following current national legislation. .. The NS2B/NS3 protease target sites of WNV L2 (SRB/ Novi Sad/12) were fused to a fluorescence quenching peptide (dark peptide) [16] and to the fluorescent protein GFP or Neptune [17], and cloned into the eukaryotic expression vector pcDNA3.1+ (Invitrogen) using third-party commercial services (GeneScript).

    Article Title: Fluorescence-based biosensors for West Nile virus detection
    Article Snippet: .. WNV lineage 1(L1) (NY99-flamingo382-99), lineage 2 (L2) (SRB/Novi Sad/12), DENV serotype 2 (DENV-2/16681/PDK53), ZIKV strains from the American ( PA259459 ), Asian (FSS13025), and African (MR766) lineages, USUV isolates (SAAR-1776 and Vienna 2001), Coxsackie B5 (CVB5), Sindbis virus (SINV) and Semliki Forest virus (SFV) were propagated and titrated on Vero-81 cells (ATCC CCL-81, Manassas, VA, USA) [ ] and used under proper BSL-2 or BSL-3 containment facilities following current national legislation. .. The NS2B/NS3 protease target sites of WNV L2 (SRB/Novi Sad/12) were fused to a fluorescence quenching peptide (dark peptide) [ ] and to the fluorescent protein GFP or Neptune [ ], and cloned into the eukaryotic expression vector pcDNA3.1+ (Invitrogen) using third-party commercial services (GeneScript).

    Article Title: Effective virus inactivation and removal by steps of Biotest Pharmaceuticals IGIV production process
    Article Snippet: Simian Virus 40 (SV 40) : SV40 was obtained from ATCC (SV40-PML 2, ATCC VR-821). .. Sindbis Virus (SinV) : Sindbis Virus (ATCC: VR-1248) was obtained from Prof. Chr. .. For virus titration CV-1 cells from ATCC (CCL-70) were used.

    Article Title: Arthritogenic Alphavirus Vaccines: Serogrouping Versus Cross-Protection in Mouse Models
    Article Snippet: Vero Specificity RRV E2 RRV Sindbis virus Barmah Forest virus Pan flavivirus Mclab G8 B82A2 2F2 10E10-C2 4G2 RRVT48 + + - - - RRVTT + + - - - BFV - - - + - SINV - - + - - KUNV - - - - + b c Supplementary Fig. 3. b Conservative amino acid differences (as defined by Miyata et al., 1979. .. J Mol Evol 12:219-36) between RRVTT and RRVT48. c Non-conservative amino acid changes between RRVTT and RRVT48. d Binding of monoclonal antibodies in fixed cell ELISAs to Sindbis virus (SINV), Barmah Forest virus (BFV) and WNV Kunjin strain (KUNV) using a panel of monoclonal antibodies; RRV-specific G8, RRV-specific B82A2, SINV-specific 2F2 (kindly provided by Dr G Burgess, James Cook University), BFVspecific 10E10-C2 (kindly provided by Dr Burgess) and the pan-flavivirus 4G2 (ATCC# HB-112). e Replication of RRVTT and RRVT48 after infection of C6/36 or Vero cells at MOI=0.01 for 1 hour followed by washing. ..

    Article Title: A potent neutralizing IgM mAb targeting the N218 epitope on E2 protein protects against Chikungunya virus pathogenesis
    Article Snippet: .. CHIKV Ross (GenBank Accession number: {"type":"entrez-nucleotide","attrs":{"text":"AF490259","term_id":"323516040","term_text":"AF490259"}} AF490259 ), kindly provided by Dr. Ooi Eng Eong from DUKE-NUS, Sindbis virus (SINV) (GenBank Accession number: {"type":"entrez-nucleotide","attrs":{"text":"NC_001547","term_id":"9790313","term_text":"NC_001547"}} NC_001547 ) and Ross River Virus (RRV) (ATCC) were also used in the PRNT. ..

    Article Title: Mosquito cells persistently infected with dengue virus produce viral particles with host-dependent replication.
    Article Snippet: .. Sindbis virus (SINV) (VR-1248 strain-ATCC) was similarly propagated. ..

    Binding Assay:

    Article Title: Arthritogenic Alphavirus Vaccines: Serogrouping Versus Cross-Protection in Mouse Models
    Article Snippet: Vero Specificity RRV E2 RRV Sindbis virus Barmah Forest virus Pan flavivirus Mclab G8 B82A2 2F2 10E10-C2 4G2 RRVT48 + + - - - RRVTT + + - - - BFV - - - + - SINV - - + - - KUNV - - - - + b c Supplementary Fig. 3. b Conservative amino acid differences (as defined by Miyata et al., 1979. .. J Mol Evol 12:219-36) between RRVTT and RRVT48. c Non-conservative amino acid changes between RRVTT and RRVT48. d Binding of monoclonal antibodies in fixed cell ELISAs to Sindbis virus (SINV), Barmah Forest virus (BFV) and WNV Kunjin strain (KUNV) using a panel of monoclonal antibodies; RRV-specific G8, RRV-specific B82A2, SINV-specific 2F2 (kindly provided by Dr G Burgess, James Cook University), BFVspecific 10E10-C2 (kindly provided by Dr Burgess) and the pan-flavivirus 4G2 (ATCC# HB-112). e Replication of RRVTT and RRVT48 after infection of C6/36 or Vero cells at MOI=0.01 for 1 hour followed by washing. ..

    Bioprocessing:

    Article Title: Arthritogenic Alphavirus Vaccines: Serogrouping Versus Cross-Protection in Mouse Models
    Article Snippet: Vero Specificity RRV E2 RRV Sindbis virus Barmah Forest virus Pan flavivirus Mclab G8 B82A2 2F2 10E10-C2 4G2 RRVT48 + + - - - RRVTT + + - - - BFV - - - + - SINV - - + - - KUNV - - - - + b c Supplementary Fig. 3. b Conservative amino acid differences (as defined by Miyata et al., 1979. .. J Mol Evol 12:219-36) between RRVTT and RRVT48. c Non-conservative amino acid changes between RRVTT and RRVT48. d Binding of monoclonal antibodies in fixed cell ELISAs to Sindbis virus (SINV), Barmah Forest virus (BFV) and WNV Kunjin strain (KUNV) using a panel of monoclonal antibodies; RRV-specific G8, RRV-specific B82A2, SINV-specific 2F2 (kindly provided by Dr G Burgess, James Cook University), BFVspecific 10E10-C2 (kindly provided by Dr Burgess) and the pan-flavivirus 4G2 (ATCC# HB-112). e Replication of RRVTT and RRVT48 after infection of C6/36 or Vero cells at MOI=0.01 for 1 hour followed by washing. ..

    Infection:

    Article Title: Arthritogenic Alphavirus Vaccines: Serogrouping Versus Cross-Protection in Mouse Models
    Article Snippet: Vero Specificity RRV E2 RRV Sindbis virus Barmah Forest virus Pan flavivirus Mclab G8 B82A2 2F2 10E10-C2 4G2 RRVT48 + + - - - RRVTT + + - - - BFV - - - + - SINV - - + - - KUNV - - - - + b c Supplementary Fig. 3. b Conservative amino acid differences (as defined by Miyata et al., 1979. .. J Mol Evol 12:219-36) between RRVTT and RRVT48. c Non-conservative amino acid changes between RRVTT and RRVT48. d Binding of monoclonal antibodies in fixed cell ELISAs to Sindbis virus (SINV), Barmah Forest virus (BFV) and WNV Kunjin strain (KUNV) using a panel of monoclonal antibodies; RRV-specific G8, RRV-specific B82A2, SINV-specific 2F2 (kindly provided by Dr G Burgess, James Cook University), BFVspecific 10E10-C2 (kindly provided by Dr Burgess) and the pan-flavivirus 4G2 (ATCC# HB-112). e Replication of RRVTT and RRVT48 after infection of C6/36 or Vero cells at MOI=0.01 for 1 hour followed by washing. ..

    Plaque Reduction Neutralization Test:

    Article Title: A potent neutralizing IgM mAb targeting the N218 epitope on E2 protein protects against Chikungunya virus pathogenesis
    Article Snippet: .. CHIKV Ross (GenBank Accession number: {"type":"entrez-nucleotide","attrs":{"text":"AF490259","term_id":"323516040","term_text":"AF490259"}} AF490259 ), kindly provided by Dr. Ooi Eng Eong from DUKE-NUS, Sindbis virus (SINV) (GenBank Accession number: {"type":"entrez-nucleotide","attrs":{"text":"NC_001547","term_id":"9790313","term_text":"NC_001547"}} NC_001547 ) and Ross River Virus (RRV) (ATCC) were also used in the PRNT. ..



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    Growth kinetics of Zambian SINV isolate M115 Vero (A) and BHK-21 (B) cells were infected with SINV isolate M115 and <t>AR339</t> at an MOI of 0.0001. C6/36 cells (C) were infected with SINV at an MOI of 0.01. Titers at the indicated each time point were determined by plaque assay. The values shown are mean ± standard deviation (SD) of triplicate. * p < 0.05, *** p < 0.001 by two-way ANOVA test.
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    Growth kinetics of Zambian SINV isolate M115 Vero (A) and BHK-21 (B) cells were infected with SINV isolate M115 and <t>AR339</t> at an MOI of 0.0001. C6/36 cells (C) were infected with SINV at an MOI of 0.01. Titers at the indicated each time point were determined by plaque assay. The values shown are mean ± standard deviation (SD) of triplicate. * p < 0.05, *** p < 0.001 by two-way ANOVA test.
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    Growth kinetics of Zambian SINV isolate M115 Vero (A) and BHK-21 (B) cells were infected with SINV isolate M115 and <t>AR339</t> at an MOI of 0.0001. C6/36 cells (C) were infected with SINV at an MOI of 0.01. Titers at the indicated each time point were determined by plaque assay. The values shown are mean ± standard deviation (SD) of triplicate. * p < 0.05, *** p < 0.001 by two-way ANOVA test.
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    A Workflow. The brain is injected with barcoded viral library. After 24–48 h of expression, during which RNA barcodes are transported to axon terminals, where they are amplified into rolonies and sequenced. B , C Single rolonies in axons have significantly weaker signals compared to somatic rolonies. B Representative image of somatic and axonal rolonies; dotted circle: somatic rolonies; arrow: axonal rolonies, with zoom-in views shown on the right; rolony intensity is color coded. Scale bar: 100 µm. C Quantification of intensity between axonal and somatic rolonies. Due to the large intensity difference between somatic and axonal rolonies, proper exposure for axonal rolonies often results in saturation of somatic rolonies. Paired t -test, two-tailed, p -value < 0.0001. D Representative images of axonal and somatic rolonies in AudC/I and ipsilateral thalamus. Images are from the first cycle of in situ sequencing. Similar results are observed across sections of the same brain regions. Dotted line, anatomical boundaries. Scale bar: top, 100 µm; bottom, 25 µm. E Registered <t>barcode</t> signals in CCFv3. Top, data in 3D model. Gray, brain outline. Bottom, coronal view of 25 µm of the sample. Gray, DAPI. F Representative images of in situ sequencing soma and a single axonal rolony with the same barcode. Soma ROI, 30.25 µm × 30.25 µm from injection site; axonal rolony ROI, 14.85 µm × 14.85 µm from ipsilateral thalamus. In total, 17 sequencing cycles are shown. G An example of tracing tracks for a single barcoded neuron reconstructed by connecting rolonies. Rolony location is indicated in white; soma location is indicated as a large green dot in ipsilateral cortex. AudC/I, contra/ipsilateral auditory cortex; Thal, thalamus; BC, barcode; D, dorsal; V, ventral; C, caudal; R, rostral; Seq, sequencing cycle.
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    Image Search Results


    Growth kinetics of Zambian SINV isolate M115 Vero (A) and BHK-21 (B) cells were infected with SINV isolate M115 and AR339 at an MOI of 0.0001. C6/36 cells (C) were infected with SINV at an MOI of 0.01. Titers at the indicated each time point were determined by plaque assay. The values shown are mean ± standard deviation (SD) of triplicate. * p < 0.05, *** p < 0.001 by two-way ANOVA test.

    Journal: Virus Research

    Article Title: Mosquito-borne alphaviruses in Zambia: Isolation and characterization of Eilat and Sindbis viruses

    doi: 10.1016/j.virusres.2025.199604

    Figure Lengend Snippet: Growth kinetics of Zambian SINV isolate M115 Vero (A) and BHK-21 (B) cells were infected with SINV isolate M115 and AR339 at an MOI of 0.0001. C6/36 cells (C) were infected with SINV at an MOI of 0.01. Titers at the indicated each time point were determined by plaque assay. The values shown are mean ± standard deviation (SD) of triplicate. * p < 0.05, *** p < 0.001 by two-way ANOVA test.

    Article Snippet: The SINV AR339 strain (VR-1248) was obtained from the ATCC.

    Techniques: Infection, Plaque Assay, Standard Deviation

    A Workflow. The brain is injected with barcoded viral library. After 24–48 h of expression, during which RNA barcodes are transported to axon terminals, where they are amplified into rolonies and sequenced. B , C Single rolonies in axons have significantly weaker signals compared to somatic rolonies. B Representative image of somatic and axonal rolonies; dotted circle: somatic rolonies; arrow: axonal rolonies, with zoom-in views shown on the right; rolony intensity is color coded. Scale bar: 100 µm. C Quantification of intensity between axonal and somatic rolonies. Due to the large intensity difference between somatic and axonal rolonies, proper exposure for axonal rolonies often results in saturation of somatic rolonies. Paired t -test, two-tailed, p -value < 0.0001. D Representative images of axonal and somatic rolonies in AudC/I and ipsilateral thalamus. Images are from the first cycle of in situ sequencing. Similar results are observed across sections of the same brain regions. Dotted line, anatomical boundaries. Scale bar: top, 100 µm; bottom, 25 µm. E Registered barcode signals in CCFv3. Top, data in 3D model. Gray, brain outline. Bottom, coronal view of 25 µm of the sample. Gray, DAPI. F Representative images of in situ sequencing soma and a single axonal rolony with the same barcode. Soma ROI, 30.25 µm × 30.25 µm from injection site; axonal rolony ROI, 14.85 µm × 14.85 µm from ipsilateral thalamus. In total, 17 sequencing cycles are shown. G An example of tracing tracks for a single barcoded neuron reconstructed by connecting rolonies. Rolony location is indicated in white; soma location is indicated as a large green dot in ipsilateral cortex. AudC/I, contra/ipsilateral auditory cortex; Thal, thalamus; BC, barcode; D, dorsal; V, ventral; C, caudal; R, rostral; Seq, sequencing cycle.

    Journal: Nature Communications

    Article Title: Massive multiplexing of spatially resolved single neuron projections with axonal BARseq

    doi: 10.1038/s41467-024-52756-x

    Figure Lengend Snippet: A Workflow. The brain is injected with barcoded viral library. After 24–48 h of expression, during which RNA barcodes are transported to axon terminals, where they are amplified into rolonies and sequenced. B , C Single rolonies in axons have significantly weaker signals compared to somatic rolonies. B Representative image of somatic and axonal rolonies; dotted circle: somatic rolonies; arrow: axonal rolonies, with zoom-in views shown on the right; rolony intensity is color coded. Scale bar: 100 µm. C Quantification of intensity between axonal and somatic rolonies. Due to the large intensity difference between somatic and axonal rolonies, proper exposure for axonal rolonies often results in saturation of somatic rolonies. Paired t -test, two-tailed, p -value < 0.0001. D Representative images of axonal and somatic rolonies in AudC/I and ipsilateral thalamus. Images are from the first cycle of in situ sequencing. Similar results are observed across sections of the same brain regions. Dotted line, anatomical boundaries. Scale bar: top, 100 µm; bottom, 25 µm. E Registered barcode signals in CCFv3. Top, data in 3D model. Gray, brain outline. Bottom, coronal view of 25 µm of the sample. Gray, DAPI. F Representative images of in situ sequencing soma and a single axonal rolony with the same barcode. Soma ROI, 30.25 µm × 30.25 µm from injection site; axonal rolony ROI, 14.85 µm × 14.85 µm from ipsilateral thalamus. In total, 17 sequencing cycles are shown. G An example of tracing tracks for a single barcoded neuron reconstructed by connecting rolonies. Rolony location is indicated in white; soma location is indicated as a large green dot in ipsilateral cortex. AudC/I, contra/ipsilateral auditory cortex; Thal, thalamus; BC, barcode; D, dorsal; V, ventral; C, caudal; R, rostral; Seq, sequencing cycle.

    Article Snippet: The sindbis virus (SINV) barcode library used in this study was generated by the MAPseq core facility at Cold Spring Harbor Laboratory.

    Techniques: Injection, Expressing, Amplification, Two Tailed Test, In Situ, Sequencing